Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Comparative assessment of poly (D,L-lactide-co-glycolide) nanoparticles modified by either cetyltrimethylammonium bromide or chitosan for plasmid DNA adsorption

Abd Almonem Doolaanea^1,2 , Nur Izzati Mansor¹, Nurul Hafizah Mohd Nor¹, Mohd Affendi Bin Mohd Shafri³, Susi Sukmasari⁴, Farahidah Mohamed^1,2,5

¹Department of Pharmaceutical Technology; ²IKOP Sdn. Bhd., Pilot Plant Pharmaceutical Manufacturing, Faculty of Pharmacy; ³Kulliyyah of Allied Health Sciences; ⁴Kulliyyah of Dentisry, International Islamic University Malaysia (IIUM), 25200 Kuantan; ⁵International Institute of Halal Research & Training (INHART), Kulliyyah of Engineering, IIUM, PO Box 10, 50728, Kuala Lumpur, Malaysia.

For correspondence:- Abd Doolaanea Email: abdalmonemdoolaanea@yahoo.com Tel:+60136238628

Accepted: 23 September 2017 Published: 31 October 2017

Citation: Doolaanea AA, Mansor NI, Nor NH, Shafri MA, Sukmasari S, Mohamed F. Comparative assessment of poly (D,L-lactide-co-glycolide) nanoparticles modified by either cetyltrimethylammonium bromide or chitosan for plasmid DNA adsorption. Trop J Pharm Res 2017; 16(10):2315-2324 doi: 10.4314/tjpr.v16i10.2

© 2017 The authors.
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Abstract

Purpose: To evaluate poly (D,L-lactide-co-glycolide) PLGA nanoparticles modified by cetyltrimethyl ammonium bromide (CTAB) or chitosan for plasmid DNA adsorption.

Methods: PLGA nanoparticles were prepared by solvent diffusion method and modified by including CTAB in the aqueous (F1) or oil phase (F2), or by including low (F3) or medium (F4) molecular weight chitosan. The nanoparticles were characterised by differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FTIR), as well as for cell toxicity, cell uptake and transfection.

Results: CTAB failed to confer positive charge on the nanoparticles. CTAB desorbed easily from F1 surface. This resulted in negative zeta potential, increased cytotoxicity as well as decreased cell uptake and transfection. In F2, CTAB was located mainly in PLGA matrix, resulting in negative charge with decreased cytotoxicity, and increased cell uptake and transfection compared to F1. On the other hand, chitosan-modified nanoparticles (F3 and F4) showed stronger interaction between chitosan and PLGA, leading to positively-charged particles, decreased cytotoxicity, as well as increased cell uptake and transfection. Amongst the four formulations, F4 exhibited the highest transfection.

Conclusion: These results should aid in understanding how PLGA nanoparticles are modified by CTAB and chitosan. Modification with chitosan yields PLGA nanoparticles with higher DNA adsorption and transfection with lower cytotoxicity.

Keywords: Chitosan, cetyltrimethyl ammonium bromide (CTAB), Nanoparticle, Poly (D,L-lactide-co-glycolide) PLGA, Plasmid DNA adsorption, Gene therapy